Barley Yellow Dwarf Virus RNA Requires a Cap-Independent Translation Sequence because It Lacks a 5′ Cap
نویسندگان
چکیده
منابع مشابه
The 3' untranslated region of tobacco necrosis virus RNA contains a barley yellow dwarf virus-like cap-independent translation element.
RNAs of many viruses are translated efficiently in the absence of a 5' cap structure. The tobacco necrosis virus (TNV) genome is an uncapped, nonpolyadenylated RNA whose translation mechanism has not been well investigated. Computational analysis predicted a cap-independent translation element (TE) within the 3' untranslated region (3' UTR) of TNV RNA that resembles the TE of barley yellow dwar...
متن کاملCrystallization and preliminary X-ray diffraction analysis of the barley yellow dwarf virus cap-independent translation element.
Barley yellow dwarf virus (BYDV) RNA lacks a 5' m(7)GTP cap, yet it is translated efficiently because it contains a 105-base BYDV-like cap-independent translation element (BTE) in the 3' untranslated region (UTR). To understand how the BTE outcompetes the host mRNA for protein-synthesis machinery, its three-dimensional structure is being determined at high resolution. The purification using tra...
متن کاملSequence and organization of barley yellow dwarf virus genomic RNA
The nucleotide sequence of the genomic RNA of barley yellow dwarf virus, PAV serotype was determined, except for the 5'-terminal base, and its genome organization deduced. The 5,677 nucleotide genome contains five large open reading frames (ORFs). The genes for the coat protein (1) and the putative viral RNA-dependent RNA polymerase were identified. The latter shows a striking degree of similar...
متن کامل5′ UTR m6A Promotes Cap-Independent Translation
Protein translation typically begins with the recruitment of the 43S ribosomal complex to the 5' cap of mRNAs by a cap-binding complex. However, some transcripts are translated in a cap-independent manner through poorly understood mechanisms. Here, we show that mRNAs containing N(6)-methyladenosine (m(6)A) in their 5' UTR can be translated in a cap-independent manner. A single 5' UTR m(6)A dire...
متن کاملSequence characterization of Barley yellow dwarf virus – OYV
Barley yellow dwarf (BYD)-associated viruses are economically important in a wide range of cereals and grasses worldwide. These viruses belong to family Luteoviridae and are efficiently transmitted by aphids in a circulative and non-propagative manner. BYD-associated viruses have a genome of single-stranded positive sense RNA (+ssRNA). Even if isolates of BYD-associated viruses from different p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Virology
سال: 1999
ISSN: 0042-6822
DOI: 10.1006/viro.1998.9507